Skip to main content

Table 1 Kinetic parameters of dog and human recombinant TK1

From: Quaternary structures of recombinant, cellular, and serum forms of Thymidine Kinase 1 from dogs and humans

  K m (μM) V max (μmol/min/mg) k cat (s -1 ) k cat /K m (M-1s-1× 10-6) n*
dTK1
dThd 0.88 ± 0.1 4.1 ± 0.1 1.81 ± 0.04 2.05 1.3 ± 0.1
AZT 0.70 ± 0.1 3.0 ± 0.1 1.32 ± 0.04 1.89 1.4 ± 0.2
ATP 331 ± 1.0 4.2 ± 0.3 1.85 ± 0.13 0.006 2.4 ± 0.4
hTK1
dThd 0.83 ± 0.2 2.5 ± 0.2 1.06 ± 0.08 1.40 1.3 ± 0.4
AZT 0.70 ± 0.2 0.95 ± 0.06 0.40 ± 0.02 0.62 1.4 ± 0.2
ATP 243 2.3 0.98 0.004 2.3
  1. Results are mean ± standard error of triplicate measurements. Data for human TK1 with ATP are from one typical experiment. * n Hill coefficient.