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Table 1 Kinetic parameters of dog and human recombinant TK1

From: Quaternary structures of recombinant, cellular, and serum forms of Thymidine Kinase 1 from dogs and humans

 

K m (μM)

V max (μmol/min/mg)

k cat (s -1 )

k cat /K m (M-1 s-1 × 10-6)

n*

dTK1

dThd

0.88 ± 0.1

4.1 ± 0.1

1.81 ± 0.04

2.05

1.3 ± 0.1

AZT

0.70 ± 0.1

3.0 ± 0.1

1.32 ± 0.04

1.89

1.4 ± 0.2

ATP

331 ± 1.0

4.2 ± 0.3

1.85 ± 0.13

0.006

2.4 ± 0.4

hTK1

dThd

0.83 ± 0.2

2.5 ± 0.2

1.06 ± 0.08

1.40

1.3 ± 0.4

AZT

0.70 ± 0.2

0.95 ± 0.06

0.40 ± 0.02

0.62

1.4 ± 0.2

ATP

243

2.3

0.98

0.004

2.3

  1. Results are mean ± standard error of triplicate measurements. Data for human TK1 with ATP are from one typical experiment. * n Hill coefficient.
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BMC Biochemistry

ISSN: 1471-2091

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