Figure 5

tyrHDL increases cell surface ABCA1. Cholesterol-loaded fibroblasts were incubated with BSA alone or with 10 μg/ml apoA-I, HDL or tyrHDL for 16 h. Total cellular ABCA1 protein was determined from whole cell lysates. Cell surface ABCA1 was determined following biotinylation and precipitation of biotinylated cell surface proteins. Total cellular and cell surface samples were run on the same 5-15% SDS-Page gradient gel followed by immunoblotting. Data are representative of three separate experiments with similar results.