Myosin-cross-reactive antigen (MCRA) protein from Bifidobacterium breve is a FAD-dependent fatty acid hydratase which has a function in stress protection

Table 1 Primers used in this work. Restriction sites are underlined

Primer	Restriction site	Resulting construct	Sequence
EMY01	PstI	pEMYL1	5 '- AACTGCAGTGGATTGTCAATTCATCCCC- 3 '
EMY02	HindIII		5' - CCCCAAGCTTGCCCGATTATGCGAACGGCT- 3'
EMY03	NdeI	pEMYC2	5 '- AACATATGTGGATTGTCAATTCATCCCC- 3 '
EMY04	AvrII		5' - CCCCCCTAGGGCCCGATTATGCGAACGGCT- 3'
BBI_fw2	NdeI	pBBI2	5 ' - AACATATGTACTACAGCAGCGGCAACTATGAG- 3 '
BBI_rv2	XhoI		5 ' - TCTCGAGTTAGATCACATGGTATTCGCGTAGCAGGGTAGC- 3 '

ISSN: 1471-2091