Figure 3

Ca²⁺influx or CoCl ₂ stimulates Bcr aggregation in cells. A) COS-1 cells transfected with the indicated plasmids were treated with DMSO (Control) or 1 μM ionomycin for 1 h. Antibodies used for Western blots are indicated to the left. B) Localization of Bcr and TG2 in HPAECs using confocal microscopy. Bar, 20 μm. C, D) HPAECs were incubated with the indicated concentrations of A23187 for 1 h (C) or CoCl₂ for 24 h (D). Antibodies used for Western blotting are indicated to the left. E) Cellular localization of Bcr in HPAECs after treatment with DMSO (Control), 20 μM A23187 for 1 h or 1000 μM CoCl₂ for 24 h using confocal microscopy. DAPI was used to stain nuclei. Arrows point to some of the intracellular Bcr aggregates. Bar, 20 μm.