Figure 7
(a) Activities of avian FAR enzymes with branched-chain acyl-CoA thioesters. The total membrane fractions of yeast cells harboring one of the avian FAR sequences were used as enzyme source and [1-14C]-labeled 2-methyl-branched acyl-CoA thioesters as substrate. Results are mean values of assays conducted in duplicate with three independent membrane preparations and represent the sum of synthesized alcohols and aldehydes. 100% correspond to the following FAR activities in pmol*min-1*mg-1 protein: AdFAR1: 25, GgFAR1: 170, TaFAR1: 200, GgFAR2: 60, TaFAR2: 18, control: 10. (b) TLC analysis of FAR assay products with branched-chain acyl-CoA thioesters. Reaction products of assays with yeast membranes harboring either GgFAR1 or GgFAR2 are shown.