Figure 6
Substrate specificities of avian FAR enzymes. In vitro assays were conducted with the total membrane fractions of yeast cells harboring one of the avian FAR sequences under optimal conditions and with [1-14C]-labeled acyl-CoA thioesters. Results are mean values of assays conducted in duplicate with three independent membrane preparations and represent the sum of synthesized alcohols and aldehydes. 100% correspond to the following FAR activities in nmol*min-1*mg-1 membrane protein: AdFAR1: 1.7, GgFAR1: 8.2, TaFAR1: 9.2, GgFAR2: 3.7, TaFAR2: 2.6, control: 0.01.