Figure 5From: Roles of quaternary structure and cysteine residues in the activity of human serine racemaseInfluence of buffer components on quaternary structure of recombinant SR. Recombinant SR purified by affinity chromatography was dialyzed against a phosphate-buffered (solid line) or Tris-buffered solution (dashed line). After concentration with Centricon filters, 50-μl aliquots of ~3 mg/ml of each recombinant SR were loaded onto a Superdex HR200 gel-filtration column and eluted with same buffer as the corresponding dialysis buffer.Back to article page