Figure 3

Stabilization of in vitro - and in vivo -formed multimers by chemical cross-linker. A. Increasing concentrations of BS³ were incubated with 12.5 μM recombinant SR on ice for 2 h, then the reactions were quenched by addition of Tris-HCl. Reaction samples were analyzed on 10% SDS-PAGE with 2.5% β-mercaptoethanol in the sample buffer. B. Increasing concentrations of protein cross-linker BS³ were incubated with lysates from mouse primary astrocytes (0.5 μg protein/μl) on ice for 2 h, and the reactions were quenched by the addition of Tris-HCl. The formation of cross-linked SR protein was analyzed by western blot with an anti-SR antibody.