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Table 1 Specificity of TaqII REase.

From: Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving

TaqII recognition sequence variant

Theoretical number of TaqII recognition sequences in bacteriophage λ DNA

Number of cleaved TaqII recognition sequences (insert clones ends analysed)

5'-GACCGA-3'

10

39

5'-CACCCA-3'

18

--

Undetermined degenerate TaqII* star recognition sequences

?

59*

  1. Determination of the TaqII recognition sequence and cleavage positions by shotgun cloning and sequencing of the resulting clones.
  2. Relaxed recognition sites were determined by shotgun cloning and sequencing of the TaqII restriction fragments obtained. After digestion, DNA was blunted with T4 DNA polymerase and cloned into the Sma I site of the pUC19 vector. TaqII 5'-GACCGA-3' and TaqII 5'-CACCCA-3', canonical recognition sequences of the enzyme as described by Barker et al. [18]; TaqII*, variants of relaxed recognition sequences.
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BMC Biochemistry

ISSN: 1471-2091

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