Figure 7
From: Assembly and proteolytic processing of mycobacterial ClpP1 and ClpP2
Proteolytic processing of ClpP1 and ClpP2 by ClpP1. (A) 2.5 μg of pure ClpP1 (lane 1), of ClpP1S98A variant (lane 2), of pure ClpP2 (lane 3), and 4.0 μg of purified ClpP2 produced in the presence of ClpP1 (lane 4) were loaded on a 15% SDS-PAGE stained with Coomassie blue. The molecular masses of the markers were indicated on the left in kDa. (B) 2.0 μg of purified ClpP2 produced in the presence of ClpP1 (lane 1) or in the presence of ClpP1S98A (lane 2), and 2.0 μg of purified ClpP2S110A purified in the presence of ClpP1 (lane 3) or in the presence of ClpP1S98A (lane 4) were loaded on a 15% SDS-PAGE stained with Coomassie blue. The molecular masses of the markers were indicated on the left in kDa.