Figure 6

Coproducing ClpP1 and ClpP2 with ClpX and ClpC ATPases did not result in Suc-LY-Amc cleavage. (A) 10 μg of total extract of cells producing ClpP1 (lane 1), ClpP1 and ClpX (lane 2), ClpP1 and ClpC (lane 3), ClpP2 (lane 4), ClpP2 and ClpX (lane5), ClpP2 and ClpC (lane 6), ClpP1 and ClpP2 without (lane 7) or with ClpX (lane 8) or ClpC (lane 9) were loaded on a 4-15% gradient SDS-PAGE stained with coomassie blue. The electrophoretic mobilities of ClpX and ClpC are indicated by asterisks. The molecular masses of the markers were indicated on the left in kDa. (B) Hydrolysis of the Suc-LY-Amc peptide in the absence (black trace) or in the presence of 10 μg of total extracts of SG1146a cells overexpressing clpP1(his) ₆ and clpX (left panel, green trace), clpP2(his) ₆ and clpX (left panel, blue trace), the clpP1-clpP2(his) ₆ operon together with clpX (left panel, red trace), clpP1(his) ₆ and clpC (right panel, green trace), clpP2(his) ₆ and clpC (right panel, blue trace), the clpP1-clpP2(his) ₆ operon together with clpC (right panel, red trace).