Figure 5

Effect of removing the putative gate on the assembly of mature ClpP1. (A) The N-terminal sequences of MTB ClpP1 (H37Rv strain, gi: 41353667) and ClpP2 (H37Rv strain, gi: 2791500) were aligned with that of E. coli ClpP (gi: 89107307). The propeptide of E. coli ClpP is written in italics. The first residues of deletion variants of E. coli ClpP are indicated in bold and by an asterisk above the sequence. The corresponding residues in MTB ClpP1 and ClpP2 sequences are written in bold and indicated with an asterisk below their sequences. (B) 200 μg of purified ClpP1 (upper panel) or of the ClpP1L16 variant (lower panel) were subjected to a size exclusion chromatography as followed by a triple detector array as described above.