Figure 2

Oligomeric assembly of recombinant ClpP1 and ClpP2. 75 μg of recombinant ClpP1 (upper panel, solid line) or ClpP2 (middle panel, solid line) produced independently or in the presence of ClpP2 (upper panel, dashed line) and ClpP1 (middle panel, dashed line) respectively were loaded on a superdex 200 10/30 column as described in the Methods section. In the lower panel, 75 μg of ClpP1 were mixed with 75 μg of ClpP2 and incubated 2 h at room temperature before being loaded on the superdex 200 column. Arrowheads indicate the elution of molecular mass standards with their molecular mass in kDa and the deduced apparent molecular masses are shown under the corresponding protein names.