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Table 1 Kinetic parameter values for 2-oxoglutarate dehydrogenase complex

From: Detailed kinetics and regulation of mammalian 2-oxoglutarate dehydrogenase

Basic kinetic parameters

Kinetic parameters for regulatory cofactors

Parameter:

Value

Sensitivity

Parameter:

Value

Sensitivity

Vmax μmol mg-1 min-1a

-

-

K aCa (μM)

0.893

0.293

K mA (mM)

0.273

3.601

K iATP (mM)

0.106

0.943

K mB (μM)

6.96

0.934

K iADP (mM)

0.305

2.073

K mC (μM)

98.6

2.839

K aMg (μM)

19.49

0.228

K ia (mM)

75.9

1.981

α C a 0

0.262

9.648

K ir (mM)

2.4

1.995

α ATP

6.694

1.628

K ic (mM)

0.112

3.970

α ADP

0.173

4.412

K iq (μM)

0.218

5.224

α Mg

1.00

6.594

K aH (pH)

6.11

3.623

β Mg

4.222

4.603

  1. a We cannot estimate the value of parameter V f , max in the model without knowing the enzyme activity in the experimental setup. The value of Vmax reported in the table is equal X × V f , max, where X is the enzyme activity. The optimization estimates that Vmax ≈ 2.16 μmol mg-1 min-1 for Figure 2, Vmax ≈ 11.033 unit/mg for Figure 3 and Figure 4, Vmax ≈ 167 nmol mg-1 min-1 for Figure 5.