Figure 3
SRC3 interacts with the carboxy terminus of 53BP1 in vitro. Various regions of 53BP1 were in vitro translated in the presence of35S-methionine and incubated with recombinant full length SRC3. The resulting complexes were isolated using FLAG Sepharose and analyzed by SDS-PAGE and fluorography. The input gel displays approximately 30% of the total amount of protein used for the interaction assay.