Figure 3

Example of cell populations with non-filamentous and filamentous cell-growth. (a)-(c) shows dotplots for the side scattering and forward scattering for cell populations harbouring the dntR gene. The more filamentous cell growth, the more scattering of light is observed, both in the forward scatter (FSC-H) and side scatter (SSC-H) channels. In (a) E.coli DH5α harbouring the one-plasmid system and in (b), E.coli DH5α harbouring the two-plasmid system with addition of only DMSO are shown. Here, the cell population divided normally, and no filamentous cell growth was observed. For the cell population in (c), E.coli DH5α harbouring the two-plasmid system with addition of DMSO and IPTG, a large subpopulation shows filamentous cell-growth. In (d) are shown fluorescence microscope images of the E.coli DH5α cells harbouring the two-plasmid system without addition of IPTG (upper picture) and with IPTG (lower picture).