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Figure 2 | BMC Biochemistry

Figure 2

From: Enzymatic synthesis of long double-stranded DNA labeled with haloderivatives of nucleobases in a precisely pre-determined sequence

Figure 2

Diagram of internally labeled native-sequence hybrid DNA molecule generation. To obtain a labeled native DNA sequence the following sequence requirements must be fulfilled: * in the seventh (X) and/or ninth position (Y) a non-A nucleotide is required depending on the labeling mode. * complementarity between the three bases within the overhang (positions 7-9; XXX/YYY) is necessary. * A/T in the tenth position is not required for a single DNA labeling. Br-U symbolizes incorporated 5-bromodeoxiuridine; N symbolizes unspecified nucleotides

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BMC Biochemistry

ISSN: 1471-2091

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