Figure 7
Gg NEU3 and Gg NEU3-Del subcellular distribution in COS7 cells. Cells were transfected with pMT21-Gg NEU3- Myc or pMT21-Gg NEU3-Del- Myc and subjected to immunofluorescence for co-localization experiments. Subcellular distribution of Gg NEU3-Myc (A, G) was related to TfR (red, merge in B) and EEA1 (red, merge in H). Subcellular distribution of Gg NEU3-Del-Myc (D, L) was also studied in relation to TfR (red, merge in E) and EEA1 (red, merge in M). Panels C, F, I, N represent a blow up of the squared regions in each B, E, H, M, respectively. Numbered white arrows evidence vesicular (1) and tubular (2) structures, as well as plasma membrane (3). Single confocal planes are shown. Scale bar: 10 μm