Figure 6

Gg NEU3 and Gg NEU3-Del distribution after Triton X-100 extraction and after Triton X-114 treatment. Detergent treatment were performed on total cell extracts derived from COS7 cells transfected with pMT21-Gg NEU3-Myc or pMT21-Gg NEU3-Del- Myc. (A) After extraction with Triton X-100 equal aliquots of the pelleted (P) and supernatant (SN) material were analyzed using anti-Myc, anti- Cav-1 and anti-TfR antibodies. Cav-1 was used as a DRM associated protein and TfR as typical membrane protein not associated to DRM. (B) After treatment with Triton X-114 identical aliquots of the aqueous [Aq] and detergent [De] phases were analyzed by Western-blot using anti-Myc, anti-HA and anti-Cav-1 antibodies. Mm NEU3-HA and Cav-1 were used as phase repartition markers. Total extract [T] analysis, prior to any treatment, is also given in both panel (A) and (B).