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Figure 5 | BMC Biochemistry

Figure 5

From: Gallus gallus NEU3 sialidase as model to study protein evolution mechanism based on rapid evolving loops

Figure 5

Gg NEU3 and Gg NEU3-Del expression in COS7 cells. Sialidase activity measured with total cell lysate from COS7 cells transfected with pMT21-Gg NEU3- Myc, pMT21-Gg NEU3-Del- Myc, pMT21 vector alone or no DNA (NTC) using 4-MU-NeuAc as substrate. (A) Sialidase specific activity in the total cell lysate. The 95% C.I. error bars indicate variation in the observed activity. (B) Sialidase specific activity over the pH range 2.5-5.5. pcDNAI-Mm NEU3- HA transfected cells were used as control. (C) Repartition of sialidase activity in the supernatant (SN) and pelleted (P) fractions obtained after 100,000 g ultracentrifugation. Values in the bar graph are expressed as percentage of the activity detectable in total extracted material.

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BMC Biochemistry

ISSN: 1471-2091

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