Figure 5From: Biochemical characterization of trans-sialidase TS1 variants from Trypanosoma congolenseTime dependence of TS reactions. Reactions of 100 μg fetuin with 100 nmol lactose (A) or 100 nmol galactose (B) were started with 50 ng TS in 50 μL and incubated at 37°C. The amount of 3'SL produced was determined by HPAEC-PAD as described under Methods. Data points are means ± standard deviations of three replicates.Back to article page