System | Km(mM) | kcat(s-1) | kcat/Km(mM-1s-1) | No. of experiments |
|---|
Mt
MetAP1a (MGMM)
| 2.85 ± 0.69 | 9.63 ± 1.42 | 3.47 ± 0.7 | 3 |
Mt
MetAP1a (MAS)
| 3.3 ± 0.35 | 11.17 ± 0.97 | 3.32 ± 0.18 | 4 |
Mt
MetAP1c (MAS)
| 1.36 ± 0.25 | 1516 ± 174 | 1156 ± 317 | 4 |
- Enzyme assays were carried out as described under 'Methods' following the incubation of purified Mt MetAP1a (1.7 nM/reaction) or Mt MetAP1c (0.038 nM/reaction) proteins with different concentrations (0.75-8 mM) of Met-Ala-Ser (MAS) or Met-Gly-Met-Met (MGMM) as substrate. Km and Vmax values were calculated from Non-linear regression analysis by fitting the data to Michaelis-Menten equation, and the results are expressed as mean ± SD. For calculating kcat values, the molecular mass of recombinant enzyme(s) was considered as 29.5 kDa (for Mt MetAP1a) or 33.1 kDa (for Mt MetAP1c). Mean ± SD for kcat/Km was determined after calculating it for individual experiment.
