Skip to main content
Figure 4 | BMC Biochemistry

Figure 4

From: Amino-terminal extension present in the methionine aminopeptidase type 1c of Mycobacterium tuberculosis is indispensible for its activity

Figure 4

Val-18 and Pro-19 at N-terminal extension are crucial for Mt MetAP1c enzyme activity. (A) Sequence alignment of MetAP1c from different gram positive bacteria. Notations used with accession number in parantheses: M tb-Mycobacterium tuberculosis (P0A5J2); M bovis-Mycobacterium bovis (P0A5J3); M avium-Mycobacterium avium (A0QJ09); M marinum-Mycobacterium marinum (B2HJQ5); M paratuberculosis-Mycobacterium paratuberculosis (Q73VS7); M leprae-Mycobacterium leprae (Q9CBU7); S coelicolor-Streptomyces coelicolor (Q9RKR2); S scabies-Streptomyces scabies (C9ZHA6); R erythropolis-Rhodococcus erythropolis (C0ZY62); N farcinica-Nocardia farcinica (Q5YSA3); G bronchialis-Gordonia bronchialis (D0LBG0); C diphtheriae-Corynebacterium diphtheriae (Q6NGL5); C glutamicum-Corynebacterium glutamicum (Q6M437); B mcbrellneri-Brevibacterium mcbrellneri (D4YNZ0); L xyli-Leifsonia xyli subsp. xyli (Q6AFH6). (B) Effect of mutations on the Mt MetAP1c enzyme activity. The indicated amounts of wild-type and mutant proteins were used to monitor the enzyme activity with 4 mM Met-Ala-Ser as the substrate. (C) Far-UV CD spectra of the different mutant proteins.

Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us