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Figure 3 | BMC Biochemistry

Figure 3

From: Comparative thermodynamic studies on substrate and product binding of O-Acetylserine Sulfhydrylase reveals two different ligand recognition modes†

Figure 3

ITC analysis of the interaction between substrate, OAS (O-acetylserine) and OASS. Data is plotted as heat signal (μJ/sec) versus time (min) in the upper panel in each fig. Lower panel-integrated heat responses per injection from panel A plotted as normalized heat per mole of injectant. The solid line represents the best fit of the data to a two independent site binding model (eqn 2). (A) Titration of St OASS (4.5 μM) with OAS (5 mM) at 25°C. (B) Titration of Hi OASS (4.5 μM) with OAS (5 mM) at 25°C. Both titrations were performed in the same buffer (20 mM Tris, pH 8.0, 50 mM NaCl).

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BMC Biochemistry

ISSN: 1471-2091

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