Figure 1

ITC data for titration of wild-type or selected alanine-substituted forms of HscB with apo-IscU. Titrations were performed in 50 mM HEPES pH 7.5, 150 mM NaCl, 4 mM TCEP at 25 °C. The concentrations of reactants were 0.25-0.4 mM HscB and 2.5-4 mM apo-IscU. Additional File 4 contains similar data for the remaining alanine-substituted forms of HscB.