Effects of additives on fibin expression in L929 and HEK293 cells. L929 or HEK293 cells were incubated with (A) 300 μM MnCl2 or (B) with either 10 mM lithium chloride, 25 μM forskolin, 10 μM dexamethasone, or 10 μM phorbol ester (final concentrations) for indicated times. Then, samples were analyzed by real-time PCR. Data are given as means ± SEM of x-fold stimulation over controls of three independent experiments using the Student's unpaired t-test. Significant differences between experimental and control conditions are marked with *p < 0.05, **p < 0.01, ***p < 0.001.