Figure 4From: Interaction of ATP with fibroblast growth factor 2: biochemical characterization and consequence for growth factor stabilityStability of FGF2 depends on the number of phosphate residues of bound stabilizers. FGF2 (5 μM) was preincubated with different stabilizers (100 μM each) at 37°C for 15 min. The complexes were then treated with trypsin (50 ng) at 37°C for 3 h. The samples were separated by SDS-PAGE and protein was visualized by Coomassie-staining.Back to article page