Western blot showing Pex5p wild-type and mutant expression levels. Upper panel - Expression levels of wild-type (WT) and mutant (R608W and D624W) forms of Pex5p in yeast cells used for two-hybrid analysis. The Gal4AD-Pex5p(C) fusions are indicated with a solid arrow. The open arrow indicates a breakdown product of the fusions and the asterisk indicates a background band recognised by the Pex5p antibody. Lower panel - Loading control, showing the same samples above probed with the anti-hexokinase antibody.