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Figure 4 | BMC Biochemistry

Figure 4

From: Aglycone specificity of Thermotoga neapolitana β-glucosidase 1A modified by mutagenesis, leading to increased catalytic efficiency in quercetin-3-glucoside hydrolysis

Figure 4

SDS-PAGE analysis. Expression of wild-type Tn Bgl1A (A.). Lane 1, MW standard; lane 2, soluble fraction at induction; lane 3; soluble fraction 1 h; lane 4, soluble fraction 2 h; lane 5, soluble fraction 3 h, lane 6, insoluble fraction 0 h; lane 7, insoluble fraction 1 h; lane 8, insoluble fraction 2 h; lane 9, insoluble fraction 3 h; lane 10, MW standard. Tn Bgl1A and some selected mutants purified by IMAC (B.). Lane 1, Tn Bgl1A; lane 2, molecular weight marker; lane 3, Tn Bgl1A-N221S/P342L; lane 4, Tn Bgl1A-F219L/K214R; lane 5, Tn Bgl1A-G222Q/V203M/K214R and lane 6, Tn Bgl1A-G222M.

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BMC Biochemistry

ISSN: 1471-2091

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