Figure 2
From: Characterization of a core fragment of the rhesus monkey TRIM5α protein
Effect of B-box 2 changes on BCCL2 expression and solubility. The wild-type (wt) BCCL2 protein and the indicated B-box 2 mutants were expressed in E. coli. The bacteria were lysed and the lysates centrifuged at 4000 × g for 10 minutes. The supernatants were loaded onto a Ni+2-NTA affinity column; the proteins eluted with 300 mM imidazole were analyzed by SDS-PAGE and Coomassie Blue staining.