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Table 2 Comparison of half-life times (t1/2) of recombinant wild-type Cc GlgP and site-directed enzyme variants in thermal denaturation experiments and in the presence of 0.4 M imidazole at 30°C

From: Orthophosphate binding at the dimer interface of Corynebacterium callunae starch phosphorylase: mutational analysis of its role for activity and stability of the enzyme

t (1/2) [min] inactivation at 45°C inactivation by imidazole at 30°C
  no Pi 10.0 mM Pi 100 mM Pi no Pi 5.0 mM Pi
wild-type 2.6 2.8 × 102 3.5 × 103 22 160
T28A 0.8 2.2 3.3 × 102 1.9 5.6
K31A 5.3 1.7 × 102 3.5 × 103 10 25
S174A 2.8 33 9.9 × 102 14 58
R141A 2.9 7.5 1.4 × 103 6.4 27
  1. The experiments were carried out in 50 mM triethanolamine buffer, pH 7.0. Values from linear regression analysis have S.D. of < 10%. Representative data were used for calculation of t1/2.