Figure 5

NMR results for N1I and N1IP. A) Histogram showing the chemical shift differences between non-phosphorylated (N1I) and phosphorylated NuMA1 peptide (N1IP) for the NH (black bars) and Hα (grey bars) protons as a function of peptide sequence. B) TOCSY and NOESY spectral regions of non-phosphorylated (red contours) and phosphorylated (green contours) NuMA1 peptides. Signals that change upon phosphorylation are indicated in both spectra. Signal labels are red for the non-phosphorylated peptide, green for the phosphorylated peptide and grey for those signals that does not change with phosphorylation. Intrarresidual signals are labelled with the proton involved in the interaction with the corresponding amide HN (greek symbol), the one letter amino acid code and the residue number. In the NOESY spectra, interresidual signals are labelled with two numbers corresponding to the aliphatic proton of residue i and the HN proton of residue i+1. N1I: sequence 1785-1810 of NuMA1, N1IP: same as N1I but phosphorylated in T6 corresponding to T1790 of NuMA1.