Figure 4
From: A sequence-dependent exonuclease activity from Tetrahymena thermophila
Cleavage and substrate specificity of telomere-specific nuclease activity. An enzyme titration (represented by triangles) using, 0, 0.46, and 2.3 ng (in A) or 0, 0.23, 0.93 ng (in B) was performed for a 20-min. incubation time. (A) Substrates (left to right) are with various repeat permutations: Ib, IVb, S9:S26*, S10:S27*, S11:S28*, S12:S29*, and S13:S30* (on a 15% urea gel). (B) Substrates (left to right) are Ib, IVb-Tetrahymena GGGGTT repeat, S14:S31*-mammal AGGGTT repeat, S15:S32*-yeast G1-3T repeat, and S8:S25*-GACGAT random repeat (on a 15% urea gel).