Figure 6
From: Regulation of Ack1 localization and activity by the amino-terminal SAM domain
Ack1 self-associates in cells. Cos7 cells were transfected with Flag-tagged wild type Ack1 alone or together with HA-tagged Ack1 NKD or KD. Ack1 was immunoprecipitated from cell lysates with anti-Flag antibodies, and the immunocomplexes were analyzed by Western blotting with anti-Flag and anti-HA antibodies. Densitometry readings of the immunoprecipitated proteins (top panels) were used to calculate the ratios of immunoprecipitated HA-Ack1 to immunoprecipitated Flag-Ack1. The HA:Flag ratio for KD was arbitrarily set to 1. Samples of whole cell lysates were analyzed for the presence of the transfected proteins (bottom panels). The figure is representative of three experiments.