Skip to main content
Figure 2 | BMC Biochemistry

Figure 2

From: Molecular characterization of tlyA gene product, Rv1694 of Mycobacterium tuberculosis: A non-conventional hemolysin and a ribosomal RNA methyl transferase

Figure 2

(A) Cloning of Rv1694. Cloning strategy of Rv1694 gene in E. coli expression vectors, pT7Nc and pET28a(+). The latter vector provides a C-terminal 6-histidine tag. (B) Purification of recombinant Rv1694: Expression of 6-histidine-Rv1694 in BL21(DE3) CodonPlus-RIPL E. coli after induction with 1 mM IPTG which was purified on Ni-NTA affinity column. Protein sample was electrophoresed on 12% SDS-PAGE and stained with coomassie blue R-250. The purified Rv1694 routinely exhibited >95% purity. (C) Immuno detection of Rv1694: Purified Rv1694 was electrophoresed on 12% SDS-PAGE and the immuno-probed with Rv1694 specific immune rabbit serum (at 1:1000 dilution) and the secondary antibody was anti-rabbit IgG HRP- antibody (1:2000 dilution). The specific protein band of Rv1694 can be seen at 30 kDa.

Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us