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Table 1 Fitting results of cell based TetX activity experimentsa

From: Characterization of tetracycline modifying enzymes using a sensitive in vivo reporter system

Enzyme

Experiment

Substrate

R 2

K m (μM)

k cat (s -1 )

[Enzyme] (μM)

K (10 -3 )

t(s)

TetX

Figure 3A

Atc

0.90

130

0.7

15

3.5

37

TetX

Figure 3B

Atc

0.96

160

0.4

15

12.2

41

TetX

Figure 3C

Dox

0.90

83

0.6

15

6.9

27

TetX

Figure 3D

ClTc

0.66

110

0.3

15

2.9

70

  1. a Fixed parameters were shown in regular fonts and values returned from fitting were shown in bold italic fonts
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BMC Biochemistry

ISSN: 1471-2091

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