Table 3 Kinetic constants of AoSOX1 on five different substrates.
Substrate | K m (mM) | V max (nkat ml -1 ) | V max /K m (s -1 ) | k cat (s -1 ) | Efficacy number (M -1 s -1 ) |
|---|---|---|---|---|---|
Glutathione | 2.78 ± 0.56 | (15.3 ± 2.89) × 107 | 55 010 | 3.60 × 106 | 1.29 × 109 |
L-Cys | 6.11 ± 0.13 | (22.2 ± 0.57) × 105 | 364 | 5.23 × 104 | 8.56 × 106 |
D-Cys | 1.55 ± 0.03 | (25.3 ± 0.02) × 104 | 163 | 5.95 × 103 | 3.84 × 106 |
DTT [thiols] | 2.41 ± 0.13 | (13.1 ± 0.22) × 106 | 5 451 | 3.09 × 105 | 1.28 × 108 |
β-mercaptoethanol | 9.73 ± 0.08 | (21.3 ± 0.05) × 105 | 219 | 5.01 × 104 | 5.15 × 106 |