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Figure 2 | BMC Biochemistry

Figure 2

From: Na+-stimulated ATPase of alkaliphilic halotolerant cyanobacterium Aphanothece halophytica translocates Na+ into proteoliposomes via Na+ uniport mechanism

Figure 2

SDS-PAGE of ATPase from Aphanothece halophytica. Samples were resolved on 12% polyacrylamide gels and were stained with Coomassie brilliant blue. Lane 1, PageRuler Prestained Protein Ladder (Fermentas); Lane 2, membrane vesicles; Lane 3, solubilized protein; Lane 4, protein from polyethylene glycol 6000 precipitation; Lane 5, purified ATPase from Superose 6. All F-type ATPase subunits were identified with reference to the previously reported F-type ATPases in Ilyobacter tartaricus [22] and Bacillus sp. strain TA2.A1 [29]. Samples in all lanes except lane 1 were precipitated with 10% TCA before SDS-PAGE. The contaminating bands were therefore unlikely due to a SDS-stable c ring.

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BMC Biochemistry

ISSN: 1471-2091

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