Figure 3

Increased stationary phase glutathionylation is a starvation response. Wild-type and petite mutant strains were grown to exponential (Exp) or stationary (Stat) phase in minimal SD media. The effect of respiratory growth (Resp) was determined by growing the wild-type strain to exponential phase on minimal media containing glycerol and ethanol as carbon sources. Carbon (-C) and nitrogen (-N) starvations were induced by growing the wild-type strain in SD media to exponential phase and shifting cells to fresh media lacking a carbon or nitrogen source, respectively. Total (GSH) and protein bound (GSSP) glutathione levels were determined. Values shown are the means of at least three independent determinations and are given in nmol/ml/A₆₀₀.