Figure 3

Partial Trp and Arg depletion of the gcn2Δ strain shows graded sensitivity of Trp and Arg availability to Gcn2p. The complete media contain 182 μg/ml Trp and 182 μg/ml Arg. (A) Trp. Growth at varying Trp concentrations (left), the tRNA^Trp charging levels 60 min after depletion (middle), time course of tRNA^Trp charging at various Trp concentration in the media (right). (B) Arg. Growth at varying Arg concentrations (left), the averaged charging levels of all four tRNA^Arg isoacceptors 60 min after depletion (middle), time course of the averaged charging levels of all four tRNA^Arg isoacceptors at various Arg concentration in the media (right).