Figure 1

Yeast growth upon single amino acid depletion shows recovery of Trp and Arg depletion dependent on GCN2. (A) Systematic depletion of amino acids from growth media in 96 well plates. Arrows indicate slow growth in Arg and Trp in the gcn2 Δ strain. (B) Recovery of growth in flasks after amino acid depletion. Depletion occurred while cells were in stationary or mid log growth. (C) Measurements of the GCN4-lacZ reporter showed that the Gcn2p-dependent translational regulation of the GCN4 mRNA is significantly reduced in the gcn2 Δ strain before and after Arg depletion. This result suggests that the level of the GCN4 protein is drastically lower in the gcn2 Δ strain as expected. Blue arrows indicate time points at which tRNA charging profiles were measured.