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Table 4 Heterologous expression of TkCPTs in Nicotiana tabacum protoplasts.

From: Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz

Enzymes

GUS activity (μ mol min-1μ g-1)

CPT activity (pmol min-1mg-1)

CPT/GUS ratio

GUS/-

1.78 (± 0.10)

-

-

GUS/TkCPT1

1.34 (± 0.16)

3.48 (± 0.38)

2.73 (± 0.50)

GUS/TkCPT2

0.97 (± 0.15)

2.05 (± 0.37)

2.28 (± 0.70)

GUS/TkCPT3

1.22 (± 0.21)

2.90 (± 0.64)

2.50 (± 0.16)

  1. Protoplasts were transfected with plasmids containing both GUS and TkCPTs or GUS alone in all cases controlled by the CaMV 35S promoter. Protoplast protein extracts were used for measurement of enzyme activities, whereas GUS activity served as the internal standard for normalization of transfection experiments. Values represent mean (± standard deviation) from six biological repetitions. No significant differences existed in enzyme activity between TkCPT1, TkCPT2 and TkCPT3, as calculated using Student's t-test (P, 0.01).
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BMC Biochemistry

ISSN: 1471-2091

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