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Table 3 Molecular mass and polydispersity of labeled and unlabeled material from ex vivo IPP incorporation.

From: Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz

rubber sample

w (10 5 Da)

n (10 5 Da)

w n -1

unlabeled 5-120 min

51.7 (± 1.34)

24.6 (± 1.42)

2.1

labeled 5-20 min

26.5 (± 1.01)

7.1 (± 0.45)

3.7

labeled 40-120 min

22.5 (± 2.27)

4.1 (± 0.30)

5.5

  1. In an ex vivo rubber biosynthesis experiment 6.5 mg of freshly prepared rubber particles was incubated with [1-14C]IPP for the time periods indicated. Extracted rubber material for each assay was fractionated by size exclusion chromatography and radioactivity levels were determined by scintillation.