Figure 6
From: Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz
Plasmids for heterologous expression of TkCPT1- 3. For heterologous expression of TkCPT1-3 in S. cerevisiae and protoplasts of N . tabacum the corresponding cDNAs were cloned into the plasmids pYEX-BX a) and pCAMBIA-1305.1 b), respectively. PCUP1, S. cerevisiae CUP1 promoter; P(A)1, cauliflower mosaic virus (CaMV) 35S polyadenylation signal sequence; URA3, S. cerevisiae URA3 locus; 35S-P, CaMV 35S promoter; GUS, beta-glucuronidase synthetic construct including catalase intron (I) (GenBank: AAK29426); P(A)2, Agrobacterium tumefaciens D-nopaline synthase polyadenylation signal sequence.