Biochemical characterization of three putative ATPases from a new type IV secretion system of Aeromonas veronii plasmid pAC3249A

Table 1 Primers used for the amplification of traE, traJ and traK genes of plasmid pAc3249A (GenBank accession number DQ890522).

Primer	Sequence (5'-3')	Target gene	Nucleotide position (in pAc3249A)
traE_f	GCCGGATCC ATGAAGCAGATAAAGCA	traE	1250 - 1266
traE_r	GCCAAGCTT ACTCACTCTTCTCTCAGTTG		3605 - 3623
traJ_f	GCCGGATCC ATGTTCCGAGAAATATT	traJ	8007 - 8023
traJ_r	GCCAAGCTT ACTTAAATTGATTCACTCAGC		9066 - 9085
traK_f	GCCGGATCC ATGAAAAATAAAGCGG	traK	9099 - 9115
traK_r	GCCAAGCTT ACaTTATTCTGATTTATCAGC		10965 - 10983

Nucleotides shown in bold indicate the restriction sites, GGATCC - Bam HI and AAGCTT - Hin dIII.

ISSN: 1471-2091