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Figure 2 | BMC Biochemistry

Figure 2

From: Biochemical characterization of three putative ATPases from a new type IV secretion system of Aeromonas veronii plasmid pAC3249A

Figure 2

12% SDS-PAGE showing the purification of the TraE, TraJ and TraK proteins. A. lane 1 - low molecular mass marker (Jena Biosciences, Jena, Germany), 2 - supernatant, 3 - lysate (E. coli XL10 pQTEV-traE), 4 - eluate of Ni-NTA column, B. lane 1 - low molecular mass marker, 2 - lysate (E. coli XL10 pQTEV-traJ), 3 - supernatant, 4 - eluate of Ni-NTA column and C. lane 1 - low molecular mass marker, 2 - supernatant, 3 - lysate (E. coli XL10 pQTEV-traK), 4 - eluate of Ni-NTA column. Mobility of TraE and TraK is in agreement with their calculated molecular mass. However, TraJ runs faster than its predicted molecular mass of 40.63 kDa.

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BMC Biochemistry

ISSN: 1471-2091

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