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Table 2 Pi-dependent activation of inactivated E. coli PEPS

From: Cloning and characterization of Escherichia coli DUF299: a bifunctional ADP-dependent kinase - Pi-dependent pyrophosphorylase from bacteria

Reaction mixtures

PEPS activity at zero time (%)

PEPS activity after 5 min (%)

PEPS activity after 10 min (%)

Complete (plus 1 mM Pi)

6

83

92

Minus 1 mM Pi

6

11

15

Plus Pi and 2 mM ADP

5

13

17

Plus Pi and 2 mM AMP

6

18

27

  1. The experiment was repeated five times; the results of one experiment, representative of all five experiments, are presented in the table.
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BMC Biochemistry

ISSN: 1471-2091

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