Figure 1

A) α-Sarcin and ricin induced cytotoxicity in HeLa cells is dose-dependent. HeLa cells were transfected with increasing amounts of pcDNA3-α-sarcin or pcDNA3-ricin, and assayed for viability, relative to a pcDNA3 control, at 48 hours post-transfection. B) HeLa cells were transfected with α-sarcin or ricin cDNA and assayed for viability, relative to a pcDNA3 control transfection, over a 48 hour time course. C) HeLa cells were transfected with increasing amounts of α-sarcin, α-sarcin-R121Q, an N-terminal 3 × Flag-tagged-sarcin, or a C-terminal mychis-tagged-sarcin and assayed for viability, relative to a pcDNA3 control, at 48 hours post-transfection. Data represents the compiled results of at least 3 independent experiments, each performed in triplicate, +/- standard deviation.