Table 2 Similarity and predicted function of PST biosynthesis genes
Gene | A. circinalis AWQC131C gene size (bp) | A. circinalis AWQC131C identity to C. raciborskii T3 | Aph. sp. NH-5 gene size bp | Aph. sp. NH-5 identity to C. raciborskii T3 | Aph. sp. NH-5/A. circinalis AWQC131C identity | Closest BLAST match | Putative function |
|---|---|---|---|---|---|---|---|
sxtC | 285 | 90% | 285 | 90% | 99% | ABI75092.1 SxtC (354 bp) | Unknown |
sxtB | 978 | 87% | 969 | 88% | 97% | ABI75093.1 SxtB | Cyclisation |
sxtA | 3705 | 90% | 3705 | 90% | 99% | ABI75094.1 SxtA | Loading of ACP, methylation, ACP, Claisen condensation |
sxtE | 477 | 64% | 363 | 83% | 75% | ABI75095.1 SxtE | Unknown |
sxtV | Disrupted | ----- | 1663 | 90% | ----- | SxtV ABI75107.1 | Inactive |
sxtD | 759 | 88% | 759 | 89% | 98% | SxtD ABI75089.1 | Desaturation |
sxtP | 1449 | 53% | 1443 | 58% | 97% | SxtP ABI75114.1 | Regulator/pilli formation |
sxtQ | 777 | 91% | 777 | 90% | 98% | SxtQ ABI75113.1 | Unknown |
sxtR | 804 | 88% | 777 | 87% | 87% | SxtR ABI75112.1 | Acyl-CoA N-acyltransferase |
sxtPER | 957 | ----- | 1059 | ----- | 82% | Nostoc punctiforme PCC 73102 ACC82015.1 | Transporter |
orf24 | 627 | 83% | 576 | 92% | 86% | Orf24 ABI75111.1 | Unknown |
sxtS | 726 | 87% | 729 | 75% | 90% | sxtS ABI75110.1 | Ring formation |
sxtT | 1020 | 82% | 1020 | 86% | 95% | sxtT ABI75109.1 | C-12 hydroxylation |
sxtU | 750 | 88% | 750 | 87% | 97% | sxtU ABI75108.1 | Reduction of C-1 |
sxtN | 870 | 86% | 846 | 89% | 92% | sxtN ABI75104.1 | Sulfotransfer |
sxtG | 1134 | 93% | 1134 | 94% | 98% | sxtG ACF94638.1 | Amidinotransfer |
sxtH | 1020 | 84% | 1020 | 87% | 95% | sxtH ACF94646.1 | C-12 hydroxylation |
sxtM | 1458 | 82% | 1458 | 76% | 92% | sxtM ABI75103.1 | Export of PSTs |
sxtI | 1839 | 90% | 1839 | 91% | 97% | sxtI ABI75099.1 | Carbamoylation |
sxtJ | 405 | 82% | 405 | 82% | 98% | sxtJ ABI75100.1 | Unknown |
sxtK | 165 | 93% | 165 | 90% | 96% | sxtK ABI75101.1 | Unknown |
sxtL | 1281 | 83% | 1278 | 85% | 92% | sxtL ABI75102.1 | Decarbamoylation |
sxtO | 570 | 12% | ----- | ----- | ----- | Cyanothece sp. PCC 8801 adenylylsulfate kinase ZP_02939438.1 | PAPS biosynthesis |
sxtW | ----- | ----- | 327 | 95% | ----- | sxtW ABI75106.1 | Ferredoxin/electron carrier |
sxtX | ----- | ----- | 756 | 87% | ----- | sxtX ABI75105.1 | N-1 hydroxylation |
orf3 | 302 | ----- | ----- | ----- | ----- | sxtN ABI75104.1 | Disrupted sulfotransferase |
orf5 | 459 | ----- | ----- | ----- | ----- | sxtN ABI75104.1 | Disrupted sulfotransferase |
orf7 | 226 | ----- | ----- | ----- | ----- | sxtN ABI75104.1 | Disrupted sulfotransferase |
orf8 | 98 | ----- | ----- | ----- | ----- | sxtW ABI75106.1 | Disrupted ferredoxin |