Figure 6
From: Dictyostelium cells bind a secreted autocrine factor that represses cell proliferation
Cells bind physiological concentrations of rAprA. Cells of the indicated strains (WT is wild-type) were incubated with different concentrations of rAprA. After 10 minutes, cells were collected and the bound rAprA was measured as in Figure 5. The plot symbols are the same as in Figure 2. Values are mean ± SEM (n = 3). For the 60 kDa rAprA, a bound rAprA value of 1.0 ng/5 × 105 cells is equivalent to 2.0 × 104 molecules/cell. The lines are curve fits to a one-site binding model with no cooperative binding.